Reactive phosphate ester of the carcinogen 2-(N-hydroxy)acetamidofluorene
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چکیده
منابع مشابه
Reactive phosphate ester of the carcinogen 2-(N-hydroxy)acetamido-fluorene.
1. Reaction of 2-(N-acetoxy)-acetamidofluorene with orthophosphate buffer at pH7 yielded a large quantity of water-soluble fluorene derivatives, which showed absorption peaks at 303, 290 and 280nm. Tris buffer under similar conditions gave negligible reaction. 2. Hydrolysis of polar material with acid or alkaline phosphatases liberated equimolar amounts of inorganic phosphate and an ether-extra...
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Context We have isolated five stable clones from a primary culture of Syrian golden hamster pancreatic duct epithelial cells and have designated them as CK1 through CK5. Design Here we describe the ability of two of these, CK1 and CK5, to metabolize the pancreas carcinogen N-nitrosobis(2oxopropyl)amine. The metabolism was assessed as the production of mutated V79 cells in a CK cell/V79 co-cultu...
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In 1960, Cramer, Miller, and Miller (1) reported the discovery of a new metabolite of the carcinogen 2-acetylaminofluorene, and from this discovery there has evolved a hypothesis concerning the mechanism of action of carcinogenic aromatic amines (2-5). This metabolite, N-hydrosy-2.acetylaminofluorene, has been found in the urine of animals fed 2-acetylaminofluorene only as a conjugate cleavable...
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متن کاملAntibodies to DNA modified by the carcinogen N-acetoxy-N-2-acetylaminofluorene.
Several studies have shown that the carcinogen N-acetoxy-N-2-acetylaminofluorene (AAAF) reacts in vivo and in vitro with native DNA and that the DNA contains a major (80%) adduct N(deoxyguanosin8-yl)-acetylaminofluorene (dGuo8-AAF) and a minor (20%) adduct 3{deoxyguanosin-N2-yl)-acetylaminofluorene (dGuo-N2-AAF) (reviewed [1,2]). It has been shown that the major alteration induced by the fluore...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1970
ISSN: 0306-3283
DOI: 10.1042/bj1200661